Figure 7.

Effect of HMWF on RAGE-NF-κB signaling in intestinal inflammation in Caco-2 cells. Caco-2 cell monolayers were pre-treated with 10 μM FPS-ZM1, a selective RAGE inhibitor before incubation with 10 μM MG-H1. (A) The protein expression of TJ markers in Caco-2 cells. (B) The protein expression of inflammatory mediators involved in RAGE signaling pathway in Caco-2 cells. The bar graph of the relative intensities of western blotting bands. (C) Localization of occludin measured by immunofluorescence. All values are presented as the mean ± SD. The data were analyzed by Dunnett’s t-test. * p < 0.05 versus the control cells, ** p < 0.01 versus the control cells, *** p < 0.001 versus the control cells, ^# p < 0.05, ^## p < 0.01 versus the MG-H1-treated cells; The localization of occludin (green) and nucleus (DAPI, blue) was determined using confocal laser scanning microscope with specific antibody; original magnification 20×; scale bar 20 μm.