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The formation of chondrocyte spheroids under a hypoxia environment in concave microwells. (A) Schematic view of the experimental setup for chondrocyte spheroid culture. (B) Fluorescent images of chondrocyte cells in 2D and 3D culture mode (i), and the comparison of gene (collagen II, collagen I, and aggrecan) expression in different culture modes (ii) [91]. ** p < 0.01. Reprinted (adapted) from [91]. Copyright (2015) with permission from Oxford University Press.
