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. 2022 Sep 17;10(9):1549. doi: 10.3390/vaccines10091549

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) Anti-IB IgG levels in sera from C57BL/6 J mice immunized with laponites LRD, HEC, and LFN), (b) Anti-IB IgG levels induced by HEC loaded with IB and its comparison with commercial adjuvants (Alum and QuilA) and PBS, (c) specific anti-IB SIgA in feces at day 49 for (a,d) specific anti-IB SIgA in feces at day 49 for (b). Symbols (*/♦/#) in (a,c) indicate differences between adjacent two groups, while symbols (*/#) in (b) indicate differences between the test laponite and commercial adjuvants (*Alum #QuilA). The cutoff was calculated as: Cut-off = mean + 10 × SD. Data are expressed as mean ± S.E.M. (n = 5). */♦ p < 0.05; **/## p < 0.01; ***/♦♦♦/### p < 0.001; ♦♦♦♦/#### p < 0.0001; and n.s. = non-significant [46].