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Western blotting analysis of mutant MexB. Sodium dodecyl sulfate–10% polyacrylamide gel electrophoresis was run with 10 μg of inner membrane protein prepared by solubilizing the crude membrane fraction with 0.8% lauryl sarcosine. The total envelope fraction of the D407E/D408E/K939R mutant (10 μg) was applied. Protein was blotted onto a polyvinylidene difluoride membrane and visualized with an antibody raised against MexB.
