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Negative ion mode nanoESI IMS CID MS/MS of the [M-4H+K]3− detected at m/z 470.714 in the MS depicted in Figure 4, corresponding to trisulfated 4,5-Δ-GlcAGalNAc[IdoAGalNAc]2. Collision energy 30 eV. For a better visualization of the fragment ions, the areas m/z 100 to m/z 350 and m/z 475 to m/z 1000 were magnified ×6 and ×10, respectively.
