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. 2022 Aug 25;12(9):788. doi: 10.3390/metabo12090788

Figure 3.

Figure 3

Lysine deficiency attenuates SCs proliferation, protein synthesis, and phosphorylation levels of the mTORC1 signaling pathway. (A) Cell proliferation; (B) representative images of cell protein synthesis levels with Western blotting; (C) representative images of protein ubiquitination level marker protein atrogin1 and MuRF1 expression levels with Western blotting; (D) representative images of phosphorylation levels of mTORC1 signaling pathway-related proteins with Western blotting. * p < 0.05, ** p < 0.01. The data shown represent mean ± SEM of three independent experiments (n = 3).