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Proteolytic (a) and lipolytic (b) activity of B. subtilis #309. (c) Gelatin zymogram shows clear proteolytic bands of protein cleavage on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the hours following B. subtilis culture conducted in the rapeseed and sunflower cake medium. Lane 1: rapeseed cake (24 h), lane 2: rapeseed cake (96 h), lane 3: molecular mass marker, lane 4: sunflower cake (24 h), lane 5: sunflower cake (96 h).
