Figure 2.

Vitamin C insufficiency and DSS treatment increase inflammation and oxidative stress. WT and KO mice were treated with DSS for 7 days. (A) Colons were stained with anti-Gr1. Scale bar, 100 μm. (B) The level of MPO was measured in homogenized colon lysates and normalized to tissue weight. (n = 15–17). ** p < 0.01. (C) The expression of 3-NT was evaluated with immunoblotting. The results are representative of three independent experiments. The concentration of vitamin C in (D) plasma (n = 8–12) and (E) colon lysates (n = 6–8) was measured using an ascorbate assay kit and normalized to colon weight. * p < 0.05, ** p < 0.01, *** p < 0.001. Statistical analysis was performed using the Tukey multiple comparisons test after one-way ANOVA.