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. 2022 Sep 12;11(18):2378. doi: 10.3390/plants11182378

Figure 7.

Figure 7

ToBRFV in seeds of systemically infected L0 cv. 6210 plants were not restricted to epidermis surface but localized in the maternal parenchyma and endothelium: (a) a bright light image of a longitudinal dissected seed; (b) higher magnification of the area marked by a box in (a); (c1–c3) seeds of ToBRFV foliar-inoculated L0 cv. 6210 (d1–d3); seeds of ToBRFV foliar-inoculated L1 cv. Lapid serving as a negative control for ToBRFV seed infection; (c1,d1) a red channel showing ToBRFV in the epidermis, parenchyma, and endothelium not entering the endosperm of L0 cv. 6210 plants and not in L1 cv. Lapid plants (the same setting used for the two images); (c2,d2) a transmitted light image; (c3,d3) merging transmitted and red channel images; (e) RT-PCR showing the presence of 1122 ToBRFV amplicon in seeds of leaf- and root-inoculated L0 cv. 6210 plants using primer set number (5); Ep, epidermis; Pa parenchyma; En, endothelium; ES, endosperm; E, embryo.