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Bile salt hydrolase (BSH) activity in Leptogranulimonas caecicola TOC12T and Granulimonas faecalis OPF53T. Full-grown cultures were streaked on a Gifu anaerobic medium (GAM) agar plate (left) and a GAM agar plate supplemented with 0.1% glycodeoxycholic acid (right). All plates were anaerobically incubated at 37 °C for 5 days. The visible precipitates surrounding colonies are the well-known indicators of bacterial BSH activity [12,27].
