Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 19;23(18):10980. doi: 10.3390/ijms231810980

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Phylogenetic analysis of LcBSH and GfBSH. Amino acid sequences of LcBSH and GfBSH proteins were aligned with previously identified bile salt hydrolases (BSHs). The phylogenetic tree was constructed with MEGA X software, using the neighbor-joining method (1000 bootstrap replications) [52]. The evolutionary distances were computed using the Poisson correction method and are in the units of the number of amino acid substitutions per site. The phylogenetic tree was displayed and customized using the online tool Interactive Tree Of Life (iTOL v6) [53]. Bootstrap values are shown by circle symbols whose size correlates with the bootstrap values. BsPVA from Lysinibacillus sphaericus was used as an outgroup.