Figure 3.

ZIKV infection induces an enrichment of flotillin-1 into raft fractions which is inhibited by AmphB treatment. Control and infected cell lysates +/− AmphB (10 µg/mL) were solubilized with 1% Triton X-100 at 4 °C and separated into membrane and cytosolic fractions by sucrose gradient centrifugation. Samples from each fraction were analysed by Western blotting using the lipid raft marker flotillin-1 and the non-raft marker TFR-1. NI: non-infected. (A) The localization of flotillin-1 in infected cells increased in raft fractions 4 and 5, whereas AmphB treatment induced a loss of flotillin-1 in the same fractions. (B) ZIKV infection and AmphB did not affect lipid raft localization of TFR-1. One representative experiment out of three performed is shown. (C) Densitometric quantification analysis of flotillin-1 (4–6) by using ImageJ software. Results are expressed as mean ± SD of three independent experiments performed. Significance was determined by the nonparametric Mann–Whitney U test using GraphPad Prism. * p value < 0.05 was considered statistically significant.