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. 2022 Sep 23;8(38):eabq0304. doi: 10.1126/sciadv.abq0304

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Fig. 4. — Protein sequences from cysteine synthases belonging to the transsulfuration pathway (CBS), sulfate assimilation pathway (CysK and CysM), O-phosphoserine pathway (CysO), and O-succinylserine pathway (Cys1a) were used for the generation of a phylogenetic tree. Primary substrates for each cysteine synthase are shown in parentheses. Sequences similar to A. loripes putative cysteine synthase Acys1a and S. pombe Cys1a (both denoted by *) were included in the analysis and either grouped within the Cys1a cluster or formed a sister clade classified as Cys1a-like. Acys1a was classified as a Cys1a-type cysteine synthase together with animal sequences from all lineages except for vertebrates, arthropods, and nematodes. The phylogenetic tree was generated with IQ-TREE (77) and visualized with iTOL (79).