TABLE 1.
Malate dehydrogenase activity of Thermus vector constructsa
| Strain | Promoter expressing mdh | MDH activity (U/mg)
|
|
|---|---|---|---|
| 25°C | 50°C | ||
| HB27 | Wild type | 2.5 ± 0.12 | 19.9 ± 0.25 |
| MM8-5/pSJ17mdhA* | J17 | 0.6 ± 0.01 | 5.5 ± 0.17 |
| HB27/pTEX1-mdh | J17 | 3.7 ± 0.06 | 29.6 ± 1.39 |
| MM8-5 | None | 0.0 | 0.5 ± 0.01 |
| MM8-5/pTEXI-mdh | J17 | 4.2 ± 0.13 | 32.6 ± 0.72 |
| MM8-5/pTEX1-D50-3 | D50-3 | 0.3 | 2.7 ± 0.07 |
| MM8-5/pTEX1-P2-300 | P2-300 | 3.0 ± 0.07 | 23.2 ± 0.99 |
a
Activity values recorded are averages of three replicate samples from three separate experiments, for a total of nine data points. Standard deviation is less than 5%. One unit of activity is defined as the amount of enzyme needed to convert 1 μmol of NADH to NAD in 1 min. *, integrative vector.