Figure 3.

Bead-based flow cytometric analysis of EV surface markers. (A) EV surface markers of plasma EV-EXÖBead^® complexes (patients: n=3 and healthy controls: n=3) are shown as reduced geometric MFI of CD9, CD63, CD81 and PD-L1 in the negative control. (B) Plot of the ratio of the MFI of PD-L1 to the MFI of CD9, the MFI of PD-L1 to the MFI of CD63, and the MFI of PD-L1 to the MFI of CD81. (C) CD9⁺ CD81⁺ CD63^Neg PD-L1⁺, CD9⁺ CD81⁺ CD63⁺ PD-L1⁺, CD9⁺ CD81^Neg CD63^Neg PD-L1⁺ and PD-L1⁺ CD9⁺ CD81^Neg CD63⁺ of plasma EV-EXÖBead^® complex were gated with FlowJo™. Significance was calculated by Two-way ANOVA with Šídák's multiple comparisons test. EV, extracellular vesicle; MFI, mean fluorescence intensity.