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. 2022 Sep 19;61(5):133. doi: 10.3892/ijo.2022.5423

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Copyright: © Benecke et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Bead-based flow cytometry analysis of EV intracellular marker and non-EV marker. (A) Intracellular EVs markers (TSG101) and non-EV markers (ApoA1) of plasma EVs-EXÖBead^® complexes and unbound plasma fraction magnetic bead complexes (n=3) are shown as reduced geometric mean fluorescence intensity in the negative control. (B) PanEV^+/Neg and ApoA1^+/Neg populations of the plasma EVs-EXÖBead^® complex and SEC (Izon qEVoriginal 70) plasma EVs-magnetic beads complex are expressed as percentages by gating with FlowJo™. Significance was calculated using an unpaired t-test. EV, extracellular vesicle.