TABLE 1.
Induction of PslaA and PlipB transcriptional luxAB gene fusions in S. liquefaciens MG1 and MG44a
| Plasmid | Specific bioluminescence (RLU/OD600 unit)b
|
||
|---|---|---|---|
| MG1 (wild type) | MG44(swrI)
|
||
| Without C4-HSL | With C4-HSL | ||
| pTO1 (PlipB::luxAB) | 11,860 ± 250 | 4,700 ± 102 | 13,650 ± 260 |
| pTO21 (PslaA::luxAB) | 4,992 ± 127 | 4,814 ± 158 | 4,962 ± 112 |
a
Bacterial cultures were grown exponentially in LB medium. Cell densities and bioluminescence were monitored along the growth curve. C4-HSL was added at a final concentration of 200 nM.
b
Results are means and standard deviations for multiple samples taken during exponential growth from at least two independent experiments. Following addition of 1 μl of n-decanal (Sigma) to 200 μl of sample, bioluminescence was quantified in a Lumat LB 9506 luminometer (EG & Berthold, Bad Wildbad, Germany). RLU, relative light units; OD600, optical density at 600 nm.