|
Construction of H. pylori strains
|
| Csd1 F |
GAGTCGTTACATTAATGTGCATATCT
|
| CcmA SDM dn R |
GCTCATTTGAGTGGTGGGAT
|
| Remove aa 2–17 F |
CAATAAAGAAAGGAGCATCAGATGGCGACTATCATCGCTC
|
| Remove aa 2–17 R |
GAGCGATGATAGTCGCCATCTGATGCTCCTTTCTTTATTG
|
| Remove aa 3–12 F |
AATAAAGAAAGGAGCATCAGATGGCAGCAAAAACAGG
|
| Remove aa 3–12 R |
CCTGTTTTTGCTGCCATCTGATGCTCCTTTCTTTATT
|
| Remove aa 119–136 F |
GGGAAACTCGCCCTAAGAATTAGGGAATGATCCAATCTAG
|
| Remove aa 119–136 R |
CTAGATTGGATCATTCCCTAATTCTTAGGGCGAGTTTCCC
|
| Remove aa 13–17 2 F |
ATAACAATAATAAATCGGCTAATGCGACTATCATCGCTCA
|
| Remove aa 13–17 2 R |
TGAGCGATGATAGTCGCATTAGCCGATTTATTATTGTTAT
|
| CcmA_40aa_linker_upstream_R |
ACCTTGTCCGCTACCCTCAAGTTTATTTTCAATTTTCTTTTC
|
| CcmA_mNeonGreen_dnstrm_F |
GATGGATGAATTATATAAATAATAGGGAATGATCCAATCTAGTCT
|
| CcmA_12_aa_link_Halo_R |
AGTACCGATTTCACTACCACCACCACTACCACCACCACTACCACCACCTTTATTTTCAAT
|
| CcmA_Halo_dnstrm_R |
AGACTAGATTGGATCATTCCCTAACCGGAAATCTCCAGA
|
| CcmA_12_aa_link_Halo_F |
ATTGAAAATAAAGGTGGTGGTAGTGGTGGTGGTAGTGGTGGTGGTAGTGAAATCGGTACT
|
| 40 aa linker halotag R |
TGGAAAGCCAGTACCGATTTCGCCTTGACCTGGGCCAGATCC
|
| 40 aa linker halotag F |
GGATCTGGCCCAGGTCAAGGCGAAATCGGTACTGGCTTTCCA
|
| C1_828 |
GATATAGATTGAAAAGTGGAT
|
| C1_829 |
TTATCAGTGCGACAAACTGGG
|
|
|
|
Cloning for protein purification
|
| CcmA_pET15b_BamHI |
GTTAGCAGCCGGATCCCTATTTATTTTCAATTTTCTTTTCTTGCTCATTGA
|
| pET15b_ccmA_delta_aa2to17_NdeI |
CGCGCGGCAGCCATATGATGGCGACTATCATCGCTCAAG
|
| CcmA_pET15b_BamHI |
GTTAGCAGCCGGATCCCTATTTATTTTCAATTTTCTTTTCTTGCTCATTGA
|
| pET15b_ccmA_delta_aa3to12_NdeI |
CGCGCGGCAGCCATATGATGGCAGCAAAAACAGGACCAG
|
| CcmA_pET15b_NdeI |
CGCGCGGCAGCCATATGATGGCAATCTTTGATAACAATAATAAATCGGCT
|
| pET15b_ccmA_delta_aa119to136_BamHI |
GTTAGCAGCCGGATCCCTAATTCTTAGGGCGAGTTTCC
|
| pET15b_ccmA_delta_aa13to17_NdeI |
CGCGCGGCAGCCATATGATGGCAATCTTTGATAACAATAATAAATCGGCT
|
|
|
|
Sequencing primers
|
|
Target
|
Primer name
|
|
|
rdxA
|
1318 |
GAAGCGGTTACAATCATCACGCCC
|
|
rdxA
|
1319 |
GCTTGAAAACACCCCTAAAAGAGCG
|
|
ccmA
|
1432 |
GATTACCATTTGCATGTAGATGGCG
|
|
ccmA
|
1433 |
CTAGAGATCTTACCATCAAGGCGC
|
|
csd5
|
Csd5-c-term-seq |
AAGCGTGAAGGTTTTAGAAATCCA
|
|
csd5
|
1194_R |
CCACAAGCTCATCATCTTCCAAAA
|
| pET15b |
T7 F |
CGAAATTAATACGACTCACTATAGG
|
| pET15b |
T7 R |
CCTCAAGACCCGTTTAGAGGCC
|
