Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Antibody	Rabbit polyclonal CEP164	EMD Millipore	ABE2621	(IF) use 1:1000
Antibody	Mouse monoclonal acetylated-α-tubulin	Cell Signaling Technology	12152	(IF) use 1:1000, (IHC) use 1:500
Antibody	Rat polyclonal ZO-1	Thermo Fisher Scientific	14-9776-82	(IF) use 1:1000
Antibody	Rabbit polyclonal DEUP1	Mercey et al., 2019. https://doi.org/10.1038/s41556-019-0427-x		(IF) use 1:1000
Antibody	Mouse monoclonal SAS6	Santa Cruz Biotechnology	sc-81431	(IF) use 1:1000
Antibody	Rabbit polyclonal PCNT	Abcam	ab4448	(IF) use 1:1000
Antibody	Mouse monoclonal FOXJ1	Thermo Fisher Scientific	14-9965-82	(IF) use 1:1000
Antibody	Rabbit polyclonal PLK4	Moyer et al., 2015. https://doi.org/10.1083/jcb.201502088		(IF) use 1:1000
Antibody	Rabbit polyclonal STIL	Moyer et al., 2015.https://doi.org/10.1083/jcb.201502088		(IF) use 1:1000
Antibody	Rabbit polyclonal CEP135	This study		Homemade, raised against a.a. 649–1140. (IF) use 1:1000
Antibody	Rabbit polyclonal acetylated-α-Tubulin	Cell Signaling Technology	5335	(IF) use 1:1000, (IHC) use 1:500
Antibody	Rabbit polyclonal CNTROB	Atlas Antibodies	HPA023321	(IF) use 1:1000
Antibody	Rabbit polyclonal CEP152	This study		Homemade, raised against a.a. 491–810. (IF) use 1:1000
Antibody	Rabbit polyclonal CDK5RAP2	Bethyl	BETIHC-00063	(IF) use 1:1000
Antibody	Goat polyclonal CEP192	Moyer and Holland, 2019.  https://doi.org/10.7554/eLife.46054		(IF) use 1:1000
Antibody	Goat polyclonal g-Tubulin	Levine et al., 2017.  https://doi.org//10.1016/j.devcel.2016.12.022		(IF) use 1:1000
Chemical compound, drug	Centrinone	Tocris Bioscience	Cat. no. 5687	Varying concentrations
Chemical compound, drug	Verapamil	Cytoskeleton, Inc	Cat. #CY-SC002	Use at 10 µM
Other	DAPI Stain	MilliporeSigma	Cat. no. 10236276001	(IF) use 1:1000