Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-HA (Mouse monoclonal) |
Sigma-Aldrich | H9658; HA-7 | WB (1:1000) |
| Antibody | Anti-HA (Mouse monoclonal) |
Covance | MMS-101R; HA.11 | WB (1:1000) |
| Antibody | Anti-GFP (Mouse monoclonal) |
Roche | 11-814-460-001 | WB (1:1000) |
| Antibody | Anti-Pgk1 (Mouse monoclonal) |
Invitrogen | AB_2532235; 22C5D8 | WB (1:1000) |
| Antibody | Anti-GFP (Rabbit polyclonal) |
Eusera | EU2 | CoIP |
| Antibody | Anti-HA (Rabbit polyclonal) |
AbCam | Ab9110 | CoIP |
| Antibody | HRP-Anti-Mouse (Goat polyclonal) |
Jackson | 115-035-146 | WB (1:20,000) |
| Other | nProtein A Sepharose 4 Fast Flow | Cytiva | 17528004 | CoIP |
| Other | Amersham Hyperfilm | GE Healthcare | 28906839 | Chemiluminescent film |
| Other | Amersham ECL | Cytiva | GERPN2209 | Chemiluminescent reagent |
| Other | Amersham ECL Prime | Cytiva | GERPN2232 | Chemiluminescent reagent |
| Other | Yeast/Fungal ProteaseArrest | GBiosciences | 786–435 | Yeast protease inhibitor; 100 X |
| Other | Concanavalin A | Sigma-Aldrich | C2010 | Yeast live-cell imaging preparation |
| Other | FM4-64 | Invitrogen | T3166 | Vacuolar Rim Stain; 4 μM |
| Other | CMAC | Setareh Biotech | 6627 | Vacuole Stain; 100 µM |
| Chemical compound, drug | Methotrexate | Enzo Life Sciences | ALX-440–045 G001 | DHFR inhibitor; 200 μg/mL, DMSO |
| Software, algorithm | CellProfiler | Lamprecht et al., 2007 | Yeast colony array image analysis | |
| Software, algorithm | MetaMorph | MDS Analytical Technologies | Version 7.8 | Automated image analysis |
| Software, algorithm | GraphPad Prism | GraphPad Software | Version 9.1.0 | Statistical analysis |
| Software, algorithm | ImageJ | NIH | Band densitometry | |
| Software, algorithm | ColabFold AlphaFold2 Advanced | Jumper et al., 2021; Mirdita et al., 2022 | Protein structure and binding prediction software |