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. 2022 Jul 27;9(27):2201300. doi: 10.1002/advs.202201300

Figure 7.

Figure 7

c‐MYC is involved in SNL‐induced microglial proliferation and neuropathic pain. A) The c‐Myc mRNA expression in naïve, sham‐, and SNL‐operated mice. *** P < 0.001, versus sham. Student's t‐test. n = 5‐8 mice per group. B) Western blot showing c‐MYC protein level in the spinal cord. ** P < 0.01, SNL versus Sham. ## P < 0.05, Nfat1 −/− versus WT, Student's t‐test. n = 3 mice per group. C) Fluorescence staining showing c‐MYC expression in the spinal cord of WT and Nfat1 −/− mice after SNL. D) Representative images of double staining of c‐MYC with IBA‐1, GFAP, and NeuN. E) Double staining of c‐MYC and NFAT1. F) Experimental timeline of procedures, treatment administration, pain behavioral testing, and tissue collection. G,H) Tamoxifen (TAM) induces mechanical allodynia (G) and thermal hyperalgesia (H) in H11‐LSL‐Myc::Cx3cr1CreERT2 mice. For mechanical allodynia, F (1, 22) = 7.614, P = 0.0114; For heat hyperalgesia, F (1, 22) = 13.61, P = 0.0013; *** P < 0.001, TAM versus vehicle. Two‐way ANOVA followed by Bonferroni's tests. n = 12 mice per group. I) TAM treatment increases the levels of Aif1 and Ki67 in the spinal cord of H11‐LSL‐Myc::Cx3cr1 CreERT2 mice. *** P < 0.001, TAM versus vehicle. Student's t‐test.