FIG. 1.

Gene organization and restriction maps of the cloned regions, including alkMa, alkMb, and rubAB operon, and a proposed model for regulation of the n-alkane hydroxylase complex by n-alkanes in Acinetobacter sp. strain M-1. Thick arrows indicate the orientation of each gene. The downstream region of alkMb was sequenced by inverse PCR (see text). The putative products of the genes are indicated below the thick arrows. Very long chain n-alkanes and long-chain n-alkanes induce alkMa and alkMb, respectively, and each of the alkane hydroxylase components forms a complex with the constitutively expressed rubredoxin and rubredoxin reductase.