FIG. 2.

Phylogenetic tree of alkane hydroxylases. Complete protein sequences of the alkane hydroxylases of several species were selected from the database and aligned using the ClustalW program. The species and proteins are Mycobacterium tuberculosis Rv3252c (accession number F70593); Pseudomonas putida AlkB (AJ233397); P. oleovorans AlkB (X65936); P. aeruginosa AE004581; P. punda xylene monooxygenase (XylM, A37316); A. calcoaceticus strain ADP1 AlkM (AJ002316); and Acinetobacter sp. strain M-1 AlkMa and AlkMb (AB049410 and AB049411, respectively). The percent identity values of the deduced amino acid sequences to AlkMa are shown in parentheses.