TABLE 1.
Plasmids used in this study
| Plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| pMX4.2 | Apr; contains 4.2-kb XbaI fragment of the gene library including alkMa and alkRa (Fig. 1) | This study |
| pME3.4 | Apr; contains 3.4-kb EcoRI fragment of the gene library (Fig. 1) | This study |
| pMC2.2 | Apr; contains 2.2-kb ClaI fragment of the gene library (Fig. 1) | This study |
| pMH2.5 | Apr; contains 2.5-kb HindIII fragment of the gene library (Fig. 1) | This study |
| pMX2.7 | Apr; contains 2.7-kb XbaI fragment of the gene library including alkMb and alkRb (Fig. 1) | This study |
| pKT231 | Kmr, Smr | 3 |
| pMFY31 | Apr, Tcr, Cmr | 6 |
| pMFYalkMa | Apr, Tcr, Cmr; contains PstI-digested pMX4.2 in the PstI site of pMFY31 | This study |
| pMFYalkMb | Apr, Tcr, Cmr; contains PstI-digested pMX2.7 in the PstI site of pMFY31 | This study |