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. 2022 Sep 22;221(12):e202112032. doi: 10.1083/jcb.202112032

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© 2022 Verweij et al.

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Figure S1. — Supplemental data related to Fig. 1. (A) Examples of DC TIRF-M analysis for EEA1-GFP in CD63-pHuji expressing HeLa. (B) Fluorescent analysis of LAMP1(-GFP) and CD63(-mRFP) localization apilimod-treated HeLa cells at different z (indicated in lower panels). (C) CD63-pHluorin fusion-spot size analysis in control (ctrl) or apilimod (apili)-treated condition. N > 100 fusion events from >10 cells in a typical experiment. Bars: 5 µm.