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. 2022 Sep 16;2022:3012218. doi: 10.1155/2022/3012218

Figure 2.

Figure 2

Lipopolysaccharide-promoted proliferation- (LPS-) induced inflammatory responses in macrophages affect A disintegrin and metalloprotease 10 (ADAM-10) and matrix metalloproteinase 12 (MMP-12). (a) The upper bar graph shows increased cell viability induced by LPS; the lower bar graph shows that MMP-12 inhibition had no significant effect on cell viability. Each group contained six samples. (b) The upper dot plot indicates LPS-inhibited ADAM-10 mRNA synthesis, determined by qualitative PCR (qPCR), with each dot representing one read. The lower bar graph shows increased secretion of ADAM-10 proteins by LPS, as determined by enzyme-linked immunosorbent assay (ELISA); each group containing four wells. (c) LPS stimulated ADAM-10 and MMP-12 mRNA synthesis in a time-dependent manner. Each dot represents a readout. (d) The effect of LPS (1 μg/ml, 12 h) on ADAM-10, MMP-12, iNOS, and TNF-α transcript levels in J774a.1 and RAW246.7 cells. Each dot represents a readout. (e) Flow cytometry analysis indicated inhibition of MMP-12 which decreased ADAM-10 expression.