Table 1.
Antimicrobial susceptibilities and molecular characteristics of recipient, donor and transconjugants
| Strain | Description | ESBL | Molecular characteristics | MICs and antimicrobial susceptibility | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTX | CAZ | CPM | P/T | CIP | AZT | |||||||||||
| Recipient | J53 | E. coli J53 | − | − | 0·12 | S | 0·12 | S | 0·12 | S | 2/4 | S | 0·12 | S | 0·06 | S |
| Donor 1 | SGP-1 | ST23 serotype K1 K. pneumoniae |
+ | CTX-M-15 | >128 | R | 64 | R | >128 | R | 8/4 | S | 2 | I | >64 | R |
| Transconjugants | JS-1 |
E. coli J53/ESBL K. pneumoniae SGP-1 |
+ | CTX-M-15 | >128 | R | 64 | R | 128 | R | 32/4 | I | 1 | S | >64 | R |
| JS-2 | + | CTX-M-15 | >128 | R | 64 | R | >128 | R | 32/4 | I | 0·5 | S | >64 | R | ||
| JS-4 | + | CTX-M-15 | >128 | R | 64 | R | 32 | R | 32/4 | S | 1 | S | >64 | R | ||
| JS-5 | + | CTX-M-15 | >128 | R | 64 | R | 32 | R | 64/4 | I | 1 | S | >64 | R | ||
| JS-6 | + | CTX-M-15 | >128 | R | 128 | R | 128 | R | 64/4 | I | 2 | I | >64 | R | ||
| JS-7 | + | CTX-M-15 | >128 | R | 32 | R | 32 | R | 16/4 | S | 1 | S | >64 | R | ||
| JS-8 | + | CTX-M-15 | >128 | R | 64 | R | 128 | R | 32/4 | I | 1 | S | >64 | R | ||
| Donor 2 | INS-2 | ST23 serotype K1 K. pneumoniae |
+ | CTX-M-15 | >128 | R | 64 | R | 128 | R | 8/4 | S | 2 | I | >64 | R |
| Transconjugants | JI-2 |
E. coli J53/ESBL K. pneumoniae INS-2 |
+ | CTX-M-15 | >128 | R | 32 | R | 128 | R | 8/4 | S | 2 | I | >64 | R |
| JI-3 | + | CTX-M-15 | >128 | R | 16 | R | 64 | R | 8/4 | S | 2 | I | >64 | R | ||
| JI-5 | + | CTX-M-15 | >128 | R | 64 | R | 16 | I | 16/4 | S | 0·12 | S | 64 | R | ||
| JI-10 | + | CTX-M-15 | >128 | R | 16 | R | 128 | R | 8/4 | S | 2 | I | >64 | R | ||
| JI-11 | + | CTX-M-15 | >128 | R | 16 | R | 128 | R | 8/4 | S | 2 | I | >64 | R | ||
| JI-15 | + | CTX-M-15 | >128 | R | 32 | R | 128 | R | 8/4 | S | 2 | I | >64 | R | ||
| Recipient | DH5α | E. coli DH5α | − | − | 0·12 | S | 0·12 | S | 0·12 | S | 0·5/4 | S | 0·12 | S | 0·06 | S |
| Transformants | FI14-1 |
E. coli DH5α/ESBL JI14 plasmid |
+ | CTX-M-15 | 64 | R | 16 | R | 4 | S | 4/4 | S | 0·25 | S | 16 | R |
| FI14-2 | + | CTX-M-15 | 64 | R | 16 | R | 2 | S | 4/4 | S | 0·25 | S | 16 | R | ||
MIC, Minimum inhibitory concentration; CTX, cefotaxime; CAZ, ceftazidime; CPM, cefepime; P/T, piperacillin-tazobactam; CIP, ciprofloxacin; AZT, aztreonam; R, resistant; I, intermediate; S, susceptible.