Table 5.

Nucleotide and amino acid changes in the E1 gene of CHIKV

Position No.	Nucleotide change observed at position no.	Amino acid substitution	Change in side chain	Change in side chain polarity	Change in side chain charge	Comment/reference
211	A631G	K211E	Basic to acidic	Basic to acidic polar	Positive to negative	Afreen et al. [10]
269	A805G	M269V	Sulfur containing to aliphatic	Non-polar to non-polar	Neutral to neutral	Shrinet et al. [14], Afreen et al. [10]
284	T852A	D284E	acidic to acidic	Acidic to acidic polar	negative to negative	Shrinet et al. [14], Afreen et al. [10]
317	A949G	I317V	Aliphatic to aliphatic	Non-polar to non-polar	Neutral to neutral	Found in this study
322	T965C	V322A	Aliphatic to aliphatic	Non-polar to non-polar	Neutral to neutral	Shrinet et al. [14], Afreen et al. [10],