Table 1.
IC50 values of MIR143#12, siR-KRAS, siR-AKT, and siR-ERK2 against each cell line at 72 h (96 h in MIR143#12-insensitive SW837 and PANC-1 cell lines).
| Cell line (mutation) | MIR143#12 | siR-KRAS | siR-AKT | siR-ERK2 | |||
|---|---|---|---|---|---|---|---|
| Colorectal | cell status for treatment | ||||||
| SW48 | 10.19 | apoptosis | >20 | >20 | >20 | ||
| WiDr | BRAF V600E | 11.17 | apoptosis | >20 | >20 | >20 | |
| HT-29 | BRAF V600E, T119S | 1.00 | apoptosis | >20 | >20 | 7.35 | |
| SW480 | KRAS G12V | 0.11 | apoptosis | 0.65 | >20 | >20 | |
| DLD-1 | KRAS G13D | 0.28 | apoptosis | 1.29 | >20 | >20 | |
| SW837 | KRAS G12C | 72 h | 316.72 | cell cycle arrest autophagy |
>20 | >20 | >20 |
| 96 h | 9.28 | cell cycle arrest autophagy |
0.32 | 4.32 | 0.26 | ||
| Pancreas | |||||||
| BxPC-3 | BRAF V487A, V492A | 0.36 | apoptosis | >20 | >20 | >20 | |
| MIA PaCa-2 | KRAS G12C | 1.30 | apoptosis | 0.08 | 5.08 | >20 | |
| PANC-1 | KRAS G12D | 72 h | 63.25 | cell cycle arrest autophagy |
6.32 | 14.66 | >20 |
| 96 h | 4.74 | cell cycle arrest autophagy |
0.2 | 0.61 | 0.98 | ||
| Normal | |||||||
| ASF-4-1 | human fibroblast | >100 | >20 | >20 | >20 | ||
| TIG-3-20 | human fibroblast | >100 | >20 | >20 | >20 | ||
| KMST-6 | human fibroblast | >100 | >20 | >20 | >20 | ||
| H9C2 | rat heart myoblast | >100 | >20 | >20 | >20 | ||