FIG. 5.
Quantitation of expression of Pspac-prsA. Whole-cell samples were prepared from late-exponential-phase cultures of IH7163 (Pspac-prsA) grown in the presence or absence of 100 μM IPTG and of IH6538, an isogenic wild-type (wt) strain. Samples corresponding to 30 μl of the cultures were subjected to SDS-PAGE (12% gel), and PrsA was analyzed by ECL immunoblotting with anti-PrsA serum. PrsA was quantitated by optical scanning using purified PrsA as a standard. The lower panel shows the integrated optical densities (IOD) of the bands in the upper panel.