Metabolic profile of C57BL/6 mice consuming a high fat diet. (A) Body weights. (B) Fat mass and (C) lean mass obtained by Echo MRI. (D) Fasting glucose and (E) fasting insulin levels after a 5 h fast. (F) Glucose tolerance test completed after a 5 h fast by intraperitoneal injection of glucose. For all measurements, C57BL/6J male mice consumed a lean control or a high-fat diet (HFD) for 15 weeks. Metabolic characterization was conducted at 14 weeks of feeding (age 20–21 weeks). Data are mean ± SEM from 9-11 mice per group for (A) through (D), 4–5 mice per group for (E), and 7–9 mice per group for (F). ∗P < 0.05, ∗∗P <0.01, ∗∗∗∗P < 0.0001 from unpaired t test for (A) through (E) and 2-way ANOVA with Šídák's multiple comparisons test for F.