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SDS-PAGE of protein fractions collected during purification containing putative benzoyl-CoA oxygenase. Lane 1, molecular mass protein standards (see Materials and Methods); lane 2, cell extract (supernatant after centrifugation at 100,000 × g; 25 μg of protein); lane 3, DEAE pool (25 μg of protein); lanes 4 to 6, Q-Sepharose active fractions (20 μg of protein); lanes 7 and 8; Cibacron blue active fractions (9 μg of protein).
