FIG. 2.

UV/visible spectra of purified benzoyl-CoA oxygenase as isolated (oxidized; ····) and after reduction with dithionite (reduced; ——). The absorption spectra of the enzyme (0.76 mg of protein/ml, corresponding to a concentration of 8.3 μM dimeric protein of 92 kDa) were recorded under anaerobic conditions in 10 mM Tris-HCl (pH 8) against buffer blank. Addition of an at least fivefold excess of dithionite resulted in complete reduction of the enzyme. The insert shows the difference spectrum of oxidized benzoyl-CoA oxygenase minus the reduced enzyme.