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Western blot with polyclonal antibodies raised against purified benzoyl-CoA oxgenase. Lane 1, purified benzoyl-CoA oxygenase; lane 2, cell extract of Thauera strain LG356; lane 3, extract of Thauera strain S2; lane 4, extract of A. evansii B5; lane 5, extract of A. evansii KB740. Approximately 30 μg of protein was applied in the case of cell extracts, and 6 μg was used in the case of the purified enzyme.
