TABLE 1.
Strains and plasmids used
| Strain or plasmid | Characteristics | Reference or source |
|---|---|---|
| Strains | ||
| P. fluorescens | ||
| WCS374 | Wild-type PGPR; Psb+ Pms+ SA+ | 22 |
| 374-05 | WCS374 Tn5 mutant; Psb+ Pms− SA− | This work |
| 374-05.1 | 374-05 Tn5 (Tcr) Psb− mutant derivative | This work |
| 374-08 | WCS374 Tn5 mutant; Psb− Pms+ SA+ | This work |
| P. putida | ||
| WCS358 | Wild-type PGPR; SA− Psb+ | 22 |
| JM218 | WCS358 Tn5 mutant; Psb− | 39 |
| E. coli | ||
| S17-1 | thi pro recA hsdR hsdM RP4-2-Tc∷Mu-Km∷Tn7 Tpr Smr | 59 |
| DH5α | recA1 endA1 φ80d lacZ dam-15 | Clontech |
| HB101 | pro leu thi lacY endA recA hsdR hsdM Smr | 10 |
| Plasmids | ||
| pLAFR1 | pRK290 containing λ cos site; Tcr Mob+ Tra− | 21 |
| pLAFR3 | pLAFR1 plus multiple cloning site of pUC8 | 59 |
| pJQ18 | pSUP5011 derivative; carries Tn5-Mob-Tc | Alfred Pühler |
| pSUP2011 | pBR325 replicon; Apr Cmr Mob; Tn5 delivery plasmid | 59 |
| pRK2013 | ColE1 replicon, helper plasmid; Kmrtra | 20 |
| pGEM-3Z | Cloning and transcription vector; Apr | Promega |
| pMB374-07 | 28-kb fragment from WCS374 cloned in pLAFR1; carries SA and pseudomonine synthesis genes | This work |
| p07H1 | 5-kb HindIII fragment from plasmid pMB374-07 cloned in pLAFR3 | This work |
| p07E34 | 8.5-kb EcoRI fragment from plasmid pMB374-07 cloned in pLAFR3 | This work |
| p07E3/p07E3R | 5-kb EcoRI fragment containing pmsCEAB genes cloned in pLAFR3 in both orientations relative to lacZ promoter | This work |
| pE3/pE3R | 5-kb EcoRI fragment containing pmsCEAB genes cloned in pGEM-3Z in both orientations relative to lacZ promoter | This work |
| pJMSal-10 | pGEM-3Z with 0.7-kb EcoRI-PstI fragment containing pmsB gene | This work |
| pJMSal-20 | pGEM-3Z with 1.7-kb EcoRI-HindIII fragment containing pmsAB genes | This work |
| pLASal-10 | pLAFR3 with 0.7-kb PstI fragment containing pmsB gene | This work |