TABLE 2.
SA production in SSM by different Pseudomonas strains (assay A) and in M9 medium by E. coli DH5α harboring different plasmids constructed in this work (assay B)
| Strain | SA (μg/ml) |
|---|---|
| Assay A | |
| WCS374 | 54 ± 25 |
| 374-05 | NDa |
| 374-05.1 | ND |
| 374-08b | 14 ± 5 |
| JM218(pLAFR1/pLAFR3) | ND |
| JM218(pMB374-07) | 3.2 ± 1.2 |
| JM218(p07E3R) | 19 ± 7 |
| JM218(p07E3) | 3.2 ± 0.7 |
| Assay B | |
| DH5α | ND |
| DH5α(pE3) | 41 ± 18 |
| DH5α(pE3R) | 61 ± 6 |
| DH5α(pE3-01) | ND |
| DH5α(pE3R-01) | 7.2 ± 1.3 |
| DH5α(pJMSal-10) | 8.8 ± 2.7 |
| DH5α(pJMSal-20) | 8.9 ± 4.6 |
a
ND, not detected.
b
Selected as representative of the WCS374 Psb− mutants.