TABLE 3.
Sequences of PX, PW and variants used in this study
| Promoter | Sequencea | IVTb | β-Galc |
|---|---|---|---|
| PX | aaTGTAACttttcaagctattcataCGACaaaaaagtgaag | X | X |
| X1 | aaTGTAACttttcaagctattcataCGTCaaaaaagtgaacg | X, w | X |
| X2 | aaTGTAACttttcaagctattcataCGTAaaaaaagtgaacg | W | NA |
| X3 | aaTGTAACttttcaagctattcataCGTAtaaaaagtgaacg | W | W |
| PW | atTGAAACcttttgaaa-cgaagctCGTAtacatacagaccg | W | W |
| W1 | atTGAAACcttttgaaa-cgaagctCGTCtacatacagaccg | X, W | X, w |
| W2 | atTGAAACcttttgaaa-cgaagctCGACtacatacagaccg | X | X, w |
a
Conserved −35 and −10 elements are in uppercase. Bases altered by mutagenesis are in bold. The experimentally mapped transcription start sites are underlined.
b
The activity of each promoter in in vitro transcription (IVT) assays is indicated. A lowercase letter indicates weak activity.
c
The activity of each promoter in vivo is summarized. NA, no activity was detected.