Table 3.
Precision and accuracy for assay of TAD quality control samples in rat plasma using the developed FPSE-UPLC-ESI-MS/MS method.
| QC sample | Intra-day (n = 5) | Inter-day(n = 3) | ||||
|---|---|---|---|---|---|---|
|
Calculated conc.(ng mL -1) (Mean ± SD) |
RSD (%) |
RE (%) |
Calculated conc.(ng mL -1) (Mean ± SD) |
RSD (%) |
RE (%) |
|
| LLOQ (0.1 ng mL -1) LQC (0.5 ng mL -1) MQC (10 ng mL -1) HQC (100 ng mL -1) |
0.098 ± 0.004 0.495 ± 0.015 10.120 ± 0.215 101.44 ± 1.66 |
4.08 3.03 2.15 1.64 |
−1.2 − 1.0 1.2 1.44 |
0.097 ± 0.005 0.488 ± 0.020 9.950 ± 0.240 101.84 ± 2.10 |
5.15 4.10 2.41 2.06 |
−2.5 − 2.4 − 0.5 1.84 |