Figure 7.

Antibody responses after LNP-CoV mRNA immunization in nonhuman primates

Cynomolgus monkeys (n = 3/group) were injected with LNP-CoV mRNA containing L202 at a dose of 10 μg (blue) or 100 μg (purple) of mRNA intramuscularly on day 0 and day 28 (arrows below the x axes indicate the day of injection). Results were compared with the antibody responses in a panel of HCS specimens (n = 36). Plasma samples were assessed using ELISA for SARS-CoV-2 S1-specific binding to IgG (A), pseudovirus neutralization (B), inhibition of ACE2 binding to RBD (C), and inhibition of ACE2 binding to the spike protein of six strains including wild-type, D614G, B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.617.2 (Delta) (D). On the day of immunization, plasma samples were collected before dose administration. Data are presented as a GMT ± geometric SD. Groups were compared by a Kruskal-Wallis test with Dunn’s multiple comparison test (B). ∗p < 0.05.