Table 1.
Primers of the detected genes
| Genes | Primers | Sequence | Accession no. |
|---|---|---|---|
| Nlrp1α | Forward | 5ʹ-ACATCCACATACTGCTCAC-3ʹ | NM_001004142 |
| Reverse | 5ʹ-ATCTTCACACCACCATCAC-3ʹ | ||
| Nlrp3 | Forward | 5ʹ-AGACCTCCAAGACCACTAC-3ʹ | BC116175 |
| Reverse | 5ʹ-ACATAGCAGCGAAGAACTC-3ʹ | ||
| Nlrp6 | Forward | 5ʹ-GGTGAAGGAGAGGAATGC-3ʹ | NM_133946 |
| Reverse | 5ʹ-GGATGAACAGTAGGCGATT-3ʹ | ||
| Nlrc4 | Forward | 5ʹ-GCTCAGTCCTCAGAACCT-3ʹ | NM_001033367 |
| Reverse | 5ʹ-GCCACCAACTTCATCTCTT-3ʹ | ||
| Aim2 | Forward | 5ʹ-CAGTTCCTCAGTTGTGGTT-3ʹ | NM_001013779 |
| Reverse | 5ʹ-GTCTAATCTTGTCTCCTTCCT-3ʹ | ||
| Asc | Forward | 5ʹ-CACAGGCAAGCACTCATT-3ʹ | NM_023258 |
| Reverse | 5’-CAGGTCAGGTTCCAGGAT-3’ | ||
| Caspase-1 | Forward | 5ʹ-GACATCCTTCATCCTCAGAA-3ʹ | NM_009807 |
| Reverse | 5ʹ-CTCCAGCAGCAACTTCAT-3ʹ | ||
| Caspase-11 | Forward | 5ʹ-CCATAGTGCTGCTCATCC-3ʹ | NM_175362 |
| Reverse | 5ʹ-CGCTGCTCCTCTACAATC-3ʹ | ||
| IL-1b | Forward | 5ʹ-CTTCAGGCAGGCAGTATC-3ʹ | NM_008361 |
| Reverse | 5ʹ-CAGCAGGTTATCATCATCATC-3ʹ | ||
| IL-18 | Forward | 5ʹ-ACTCTTGCGTCAACTTCA-3ʹ | BC024384 |
| Reverse | 5ʹ-GTCCTCTTACTTCACTGTCT-3ʹ | ||
| GAPDH | Forward | 5ʹ-TCTCCTGCGACTTCAACA-3ʹ | GU214026 |
| Reverse | 5ʹ-TGTAGCCGTATTCATTGTCA-3ʹ |