Fig. 5.

Additional features for TMM classification of ambiguous neuroblastoma cases. A Heatmap showing the telomere content of distinct telomere variant repeat types in neuroblastoma samples (n = 68). B Log₂ singleton ratio for TTTGGG and C TGAGGG singletons, normalized by telomere content, in TMM defined subgroups. Kruskal–Wallis test and Dunn’s multiple comparison test were used for statistical analysis; ambiguous cases were excluded. Whiskers are limited to 1.5 × interquartile range. D Telomere insertions in TMM defined subgroups. Kruskal–Wallis test and Dunn’s multiple comparison test were used for statistical analysis; ambiguous cases were excluded from statistical analysis. No., number; amb., ambiguous cases. E TMM classification of seven ambiguous neuroblastoma cases that could not be classified by standard criteria. High TERRA expression was defined as equal or greater than the mean TERRA expression in ALT-positive neuroblastomas. TVR singleton depletion was defined as equal or smaller than the mean singleton ratio in ALT-positive neuroblastomas. Tumors were classified depending on the given TMM associated features into TEL^[+] or ALT^[+] subgroups. The clinical course was not considered for classification and is displayed for information only