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. 2022 Sep 24;19:234. doi: 10.1186/s12974-022-02581-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Clec7a mRNA expression in control (CM); plaques-distant (PCM) and plaque-associated (PAM) microglia. Microglia detection (GFP, green), Thiazine Red (TR) staining for amyloid plaques (red) and Cst7 mRNA expression (purple) were combined on brain slices in A APP/PS1^Tg/0: CX3CR1^+/eGFP or E CX3CR1^+/eGFP mouse. A 12-mo APP/PS1^Tg/0:CX3CR1^+/eGFP mouse cortical field exhibiting amyloid plaques. Plaque-associated microglia (PAM) are located within 70 µm (purple dotted circle) of the plaque epicenter; while plaque-distant microglia (PCM) are located farther than 100 µm (orange dotted circle) from the plaque epicenter. Clec7a mRNA expression is absent from control microglia (E) but can be evidenced in both plaque-associated (A, C) and plaque-distant (A, D) microglia. White arrowheads point to Clec7a positive plaque-distant microglia. B Pseudocounts of Clec7a expression in CM (greens), PCM (oranges) and PAM (violets) along ages. C Zoomed region around TR+ plaque showing Clec7a overexpression in PAM. Note the heterogeneity for Clec7a expression among PAM, blue arrowhead for no expressing microglia, yellow arrowhead for low expressing microglia, grey arrowhead for high expressing microglia. D Zoomed region of Clec7a positive plaque-distant microglia. Scale bar 20 µm