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. 2022 Jan 26;50(17):9601–9620. doi: 10.1093/nar/gkac003

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Global histone hyperacetylation improves early G1 heterochromatin MCM loading. (A) Representative immunoblot of histone H3 acetylation in untreated and TSA-treated asynchronously proliferating RPE1-hTert cells (expressing PCNA-mTurquoise and DHB-mCherry). Cells were treated with the indicated concentrations of TSA for 3 h. Quantification of total loaded MCM3 concentration (B), and the ratio of loaded MCM3 concentration in heterochromatin to euchromatin (C) in early G1 cells that had been treated with 300 nM TSA for 3 h. Three replicates are shown. One-way ANOVA test and n (number of cells) is indicated in the figure. ^****P ≤ 0.0001.