Figure 2.

RNA editing by PPR56 and PPR65 constructs of their co-provided native targets in HeLa cells. RNA editing of the respective targets ccmFCeU103PS and nad4eU272SL, respectively, was determined from bulk sequencing of RT-PCR products after transfection of the differently tagged PPR56 and PPR65 constructs (see Figure 1B) into HeLa cells and incubation for 20 h. (A) Observed RNA editing efficiencies varied widely and reached up to 58% for the HA-His₆-MBP-PPR56 construct. Data are based on a minimum of three biological replicates (independently transfected cells) for each construct (Supplementary Table S2) (B) Approximate transfection efficiencies (%TE) were determined from the ratio of immuno-stained HA- and EYFP-positive cells (IS) to DAPI-signals. Shown are example images, scale bar: 50 μm. For more extensive documentation see Supplementary Figure S1.