Figure 3.

siRNA mediated knockdown of β-del TERT partially restores NO bioavailability in CAD. Representative images showing siRNA targeted β-del TERT (A) or total TERT (recognized all isoforms) from (B) knockdown and control siRNA treated vessels. β-del TERT in part restores NO-mediated dilation to flow (L-NAME inhibitable) in vessels from subjects with CAD am H₂O₂ (Peg-catalase inhibitable) dilation was reduced (C). Endothelium-independent dilation to papaverine was not impaired (E). siRNA to β-del TERT increased flow-induced NO production while reducing H₂O₂ (D). CTRL siRNA had no effect on dilator function or NO/H₂O₂ production (not shown). * P < 0.05 2-way ANOVA RM, N = 7–8 for C.* P < 0.05 unpaired t-test, N = 5–8 for D and E.