Table 2.
Telomerase Modulators
| Name | Order Infomation | Effect |
|---|---|---|
| AGS 499 | Gift from Dr. Ester Priel, B.G. NEGEV TECHNOLOGIES | Telomerase transcriptional activator1 |
| Control siRNA | Ambion, Silencer Negative Control siRNA #1, Cat. No. AM4611 | Control siRNA without specific target in human genome |
| siRNA TERT | Ambion Cat. No. 4427037-s372 | Genetic knock down of telomerase reverse transcriptase FL protein |
| siRNA β-del TERT | Ambion Custom siRNA Sense—UCAAGAGCCACGUCCUACGttAntisense—CGUAGGACGUGGCUCUUGAag | Genetic knock down of telomerase reverse transcriptase β-del TERT splice variant specific |
| BIBR 1532 | Tocris Bioscience Cat. No. 2981 | Selective, small molecule telomerase inhibitor |
| Lenti β-del TERT | Generated by MCW/Versity Hybridoma Core | Dominant negative splice variant of TERT lacking exon 7–8 and containing premature stop codon. |
| Lenti MTS mutant TERT-GFP | Mutation of mitochondrial target sequence R3E/R6E resulting in loss of TERT mitochondrial localization2 | |
| Lenti NLS mutant TERT-GFP | Deletion of nuclear localization signal (mutant 7A) resulting in loss of TERT nuclear localization9 | |
| NLS mimic | RRRGGSASRSLPLPKRPRR | Mimics nuclear localization signal around amino acid S227 |
| NLS mimic—A | RRRGGAASRSLPLPKRPRR | Negative control for NLS mimic; mutated equivalent of S227 to A (not phosphorlatable) |
| MTS mimic | MPRAPRCRAVRSLLRSHYRE | Mimics mitochondrial localization signal amino acid R3 and R6 |