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. 2022 Jun 18;50(17):e100. doi: 10.1093/nar/gkac521

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Co-localization of LEDGF/p75 and p52 with different epigenetic modifications. (A) Number of co-localizing LEDGF spots with H3K36me3 was measured in HeLaP4 cells (LEDGF WT – LEDGF/p75 KD – LEDF BC). (B) Co-localization ratio of LEDGF with H3K27me3 – H3K9/14ac – H3K36me3 – H3K36me2 marks. Statistical analysis was performed by a one-way ANOVA: (ns) non-significant; ***P-value < 0.001; number of cells (n) = 50 with each grey dot representing one cell and two different samples analyzed per condition. Co-localization analysis was performed on Z-stacks of expanded cells consisting of 9–11 Z-slices with a Z-step size of 0.2 μm.