FIG. 5.

Affinity blotting of a known R. flavefaciens enzyme construct with a recombinant ScaA cohesin. A GST-XynD fusion protein (molecular weight, 78,953) was subjected to SDS-PAGE (Gel), and the proteins were transferred to a PVDF membrane (Blot), and probed with ScaA-Coh2 as described in the legend to Fig. 4. Molecular mass markers (in kilodaltons) are shown to the left of the gel.