. Author manuscript; available in PMC: 2022 Sep 24.

Published in final edited form as: Curr Protoc Microbiol. 2020 Dec;59(1):e119. doi: 10.1002/cpmc.119

Table 2.

Troubleshooting Guide for the ExoV Assay

Problem	Possible cause	Solution
rDNA resistant fraction >10%	ExoV activity may have deteriorated during storage	Replace enzyme and ATP
	Presence of inhibitors that affect ExoV activity	Reisolate sample DNA
UMSCC47 resistant fraction >10%	ExoV activity may have deteriorated during storage	Replace enzyme
	Presence of inhibitors that affect ExoV activity	Reisolate sample DNA
	Presence of contaminating circular DNA	Reisolate sample DNA
HPV DNA resistant fraction >100%	Contamination in PCR DNA may not be well purified	Reisolate sample DNA Reisolate sample DNA

ExoV, exonuclease V; HPV, human papillomavirus.