TABLE 2.

Enantioselective oxidation of chiral alcohols by homogeneous THFA-DH from R. eutropha strain Bo^a

Substrate	Kmb (mM)	Vmax (U/mg)	Kcat/Km × 104 (M−1 s−1)	Ki (mM)	E
(±)-2-Butanol	1.12	28.2	3.1	—
R-(−)-2-Butanol	1.14	17.5	1.9	—
S-(+)-2-Butanol	0.59	32.0	6.6	—	3.5
(±)-2-Heptanol	0.058	96.8	204.2	1.03
R-(−)-2-Heptanol	1.16	22.1	2.3	0.82
S-(+)-2-Heptanol	0.046	131.6	350.0	0.93	150.2
(±)-2-Octanol	0.076	80.4	129.4	1.34
R-(−)-2-Octanol	0.269	18.2	8.3	6.62
S-(+)-2-Octanol	0.051	101.3	243.0	0.98	29.4
(±)-Solketal	ND
R-(−)-Solketal	0.11	3.1	3.4	—
S-(+)-Solketal	0.74	5.6	0.9	—	0.27

^aThe kinetic parameters were determined using ferricyanide as an artificial electron acceptor and under the conditions described in Table 1, footnote a. The enantiomeric ratios are expressed in E values as described in Materials and Methods. —, no substrate inhibition detected under the conditions used. 

^bThe curves were fitted to the model of Michaelis and Menten with or without substrate inhibition. ND, not determined.